Table 1 RT‒qPCR system
Components | Volume |
|---|---|
cDNA | 2 μL |
Forward Primer(10 μM) | 1 μL |
Reverse Primer(10 μM) | 1 μL |
2 × TransStart Top Green qPCR SuperMix | 10 μL |
ddH2O | 6 μL |
Total Volume | 20 μL |
Components | Volume |
|---|---|
cDNA | 2 μL |
Forward Primer(10 μM) | 1 μL |
Reverse Primer(10 μM) | 1 μL |
2 × TransStart Top Green qPCR SuperMix | 10 μL |
ddH2O | 6 μL |
Total Volume | 20 μL |