Fig. 3

Cell neovascularization chips Channel 1 and channel 5 are the culture medium channels that can not only ensure the supply of nutrients but also reduce the impact of fluid flow on cells. Passage between the small rectangular structure can guarantee the precise localization of fluid infusion, can not only realize physical isolation of different kinds of cells, and can realize the mutual communication between cells, conducive to control the position of the gel distribution at the same time, in ensuring that the channel can be completely filled with liquid limit contents as needed in the channel at the same time, not easy to overflow. The matrix collagen solution was injected into channel 3 to fill the channel and act as a barrier to separate the two types of cells. HepG2 cells were encapsulated by Matrigel and injected into channel 2 to establish a 3D growth condition, and HUVECs were distributed in channel 4 as a 2D monolayer of adherent growth