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Table 5 Larvicidal properties of leaf EOs from O. americanum and O. basilicum against the larvae of A. aegypti after 12 and 24 h of exposure

From: Chemical compositions, antioxidant, antimicrobial, and mosquito larvicidal activity of Ocimum americanum L. and Ocimum basilicum L. leaf essential oils

Sample

Mosquito life stages

Time

Concentration (ppm)

% mortality ± SD

LC50 (LCL-UCL)a

LC90 (LCL-UCL)a

χ2 (df = 4)b

O. americanum

Second instar

After 12 h

25

23.57 ± 0.42

   
   

50

33.88 ± 0.46

160.34

627.43

 
   

100

45.00 ± 1.00

(86.76–217.58)

(457.69–1202.18)

 
   

200

55.80 ± 0.83

  

5.406

   

400

74.60 ± 1.14

   
  

After 24 h

25

23.56 ± 0.12

   
   

50

47.00 ± 0.69

87.96

439.54

10.45*

   

100

64.30 ± 1.02

(12.70–163.11)

(292.16–716.18)

 
   

200

80.00 ± 1.50

   
   

400

91.54 ± 1.56

 

Third instar

After 12 h

25

11.80 ± 0.89

   
   

50

31.91 ± 1.53

134.54

1119.07

2.34

   

100

45.00 ± 1.00

(46.72–227.60)

(781.28–2828.96)

 
   

200

57.80 ± 1.30

   

400

73.60 ± 0.89

  

After 24 h

25

21.20 ± 1.30

   
   

50

44.56 ± 1.28

   
   

100

58.20 ± 1.30

73.73(16.54–148.55)

577.28(353.49–961.49)

2.39*

   

200

75.60 ± 0.54

   

400

82.60 ± 0.89

O. basilicum

Second instar

After 12 h

25

32.20 ± 0.44

   
   

50

44.60 ± 1.14

61.14

1114.68

2.18

   

100

53.20 ± 1.09

(17.76–342.99)

(242.16–3126.01)

 
   

200

67.20 ± 0.83

   
   

400

76.40 ± 1.14

  

After 24 h

25

41.60 ± 1.14

   
   

50

62.80 ± 1.92

   
   

100

71.40 ± 1.19

37.14

286.55

1.22*

   

200

87.40 ± 1.14

(0.08–137.77)

(30.43–548.49)

 
   

400

93.20 ± 0.83

 

Third instar

After 12 h

25

27.40 ± 1.24

   
   

50

34.00 ± 0.87

   
   

100

56.76 ± 0.69

92.65

950.72

1.56

   

200

69.10 ± 1.88

(0.03–259.13)

(495.34–3371.99)

 
   

400

78.00 ± 1.00

  

After 24 h

25

42.35 ± 1.20

   
   

50

64.50 ± 1.57

   
   

100

79.25 ± 1.65

31.43

233.43

1.09*

   

200

86.00 ± 1.72

(0.41–111.35)

(37.22–459.36)

 
   

400

95.50 ± 1.89

  1. LCL Lower confidence level, UCL Upper confidence level
  2. a95% confidence interval
  3. bDegrees of freedom; χ2 chi-square value
  4. *Significance at P ≤ 0.05