Table 8 IC₅₀ cytotoxic concentration (μg/ml) on WI, HepG2 cells after 48 h and 72 h incubation and on MDA and MCF7 after 72 h incubation

CS-NPs

11,114 ± 3.1

5686 ± 2.5

2748 ± 2.5

921.9 ± 2.4

179.7 ± 2.6

144 ± 1.1

CAF

7565 ± 4.0

5568 ± 3.8

813.3 ± 2.3

317.7 ± 2.2

389.5 ± 1.5

120.6 ± 0.6

Folic

944528 ± 5.2

6070 ± 4.9

743.1 ± 1.3

559.1 ± 5.0

233.5 ± 1.2

261.1 ± 1.0

D4

698750 ± 8.5

34,047 ± 4.2

1082 ± 3.0

652 ± 4.0

204.5 ± 0.7

247.2 ± 1.2

D7

68962 ± 5.6

3110 ± 5.8

1123 ± 7.8

982 ± 1.1

347.3 ± 1.0

305.1 ± 2.0

MTX

725.2 ± 2.9

11.95 ± 0.3

9.764 ± 0.2

8.489 ± 0.2

20.96 ± 0.6

9.22 ± 0.1