BMC Complementary Medicine and Therapies

Table 3 Forward and reverse primer sequence for candidate genes

From: Caffeine-folic acid-loaded-chitosan nanoparticles combined with methotrexate as a novel HepG2 immunotherapy targeting adenosine A2A receptor downstream cascade

Gene	Forward primer (^/5 ––– ^/3)	Reverse primer (^/5 ––– ^/3)
*Bax*	TGCTTCAGGGTTTCATCCAG	GGCGGCAATCATCCTCTG
*Bcl-2*	AGGAAGTGAACATTTCGGTGAC	GCTCAGTTCCAGGACCAGGC
*FoxP3*	TCATCCGCTGGGCCATCCTG	GTGGAAACCTCACTTCTTGGTC
*CD73 (NT5E)*	ATTGCAAAGTGGTTCAAAGTCA	ACACTTGGCCAGTAAAATAGGG
*CD39 (ENTPD1)*	GAAGGTGCCTATGGCTGGATTAC	TGTTGGTCAGGTTCAGCATGTAG
*A2AR (ADO)*	AGCTGAAGCAGATGGAGAGC	AGGGATTCACAACCGAATTG
*B-actin*	GGATGCAGAAGGAGATCACTG	CGATCCACACGGAGTACTTG

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ISSN: 2662-7671

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