Fig. 4

Antiseizure effects of BO at 300 mg/kg in mice pre-treated with single antibiotics (van, met, amp, and neo) compared to BO at 300 mg/kg in naive mice (no antibiotic treatment). Assessment was based on seizure latency (A), frequency (B), and duration (C) induced by PTZ. The antiseizure effects were reversed following antibiotic pre-treatment. The data are shown as group mean ± SEM (n = 7). * denotes P < 0.05, *** denotes P < 0.001 (one-way analysis of variance followed by Tukey's multiple comparisons test)