Fig. 5

Effect of the Ut aqueous extract against the PA cytotoxicity in C2C12 myoblasts. Cells were treated with the 250 μg/ml Ut aqueous extract in preventive or therapeutic conditions, for 2 or 6 h, and incubated with 100 or 500 μM PA, for 24 h. Cell viability was assessed by flow cytometry using propidium iodide. 10.000 events were acquired per sample. The data are presented as the mean ± SE of three individual experiments in duplicates and the values are expressed as the percentage in relation to the control. * Indicates difference compared to the control with only PA; # indicates difference between treatments with Ut extract for 2 or 6 h; ∆ indicates difference in comparison to cultures incubated with only ethanol (p < 0.05)