Phytochemical analysis, in vitro and in silico effects from Alstonia boonei De Wild stem bark on selected digestive enzymes and adipogenesis in 3T3-L1 preadipocytes

Table 3 Calculated IC50 values of fractions on α-amylase, lipase and α-glucosidase activities

Alstonia boonei fractions	α-amylase		Pancreatic lipase		α-glucosidase
Alstonia boonei fractions	Inhibition (%)	IC50 (μg/mL)	Inhibition (%)	IC50 (μg/mL)	Inhibition (%)	IC50 (μg/mL)
ME	40.37 ± 2.43	-	41.23 ± 1.85	-	32.56 ± 5.28	-
CFF	44.64 ± 4.32	-	37.98 ± 1.53	-	45.92 ± 5.06	-
CAF	74.54 ± 1.40	1203 ± 0.364	65.42 ± 2.19	2.77 ± 0.063	42.49 ± 1.63	-
CSF	74.97 ± 3.19	206 ± 0.103	60.21 ± 4.16	25.37 ± 0.062	77.95 ± 4.29	11.97 ± 0.081
Orlistat	NA	NA	93.18 ± 2.97	0.068 ± 0.063	NA	NA
Acarbose	83.18 ± 2.97	64.58 ± 0.071	NA	NA	76.70 ± 2.63	2.84 ± 0.087

Alstonia boonei fractions: ME methanol extract, CAF crude alkaloid fraction, CSF crude saponin fraction. Results of inhibition (%) are demonstrated as Mean ± SD of 3-replicates of every fraction. IC50 values are mean ± SEM; (–) fractions/extract did not inhibit 50% of enzyme activity at 1000 μg/mL; NA not applicable

ISSN: 2662-7671