Fig. 3
Determination of CC50 and anti-hRSV activity. (A) Different fractions from EPS were incubated in different concentrations with Hep-2 cells. The cellular viability was evaluated by MTT, and CC50 was calculated by linear regression. (B) Anti-hRSV from different EPS fractions was tested using a lower CC50 concentration than that obtained in Fig. 3A. Just EPS concentration that resulted in the maximum cellular viability is shown in the graphic: LaEPS (7 µg/ml), LaEPS-C (3.4 µg/ml), LLaEPS-C (0.7 µg/ml) and BLaEPS-C (0.4 µg/ml). Cells incubated with culture medium (CC), only virus (CV) or antiviral drug Ribavirin (Ribav) were used as control