Fig. 6

Blockade of the Akt/mTOR pathway reduced GSIS restored by APS in LPS-stimulated INS-1 cells. INS-1 cells were pretreated as indicated for 24 h, starved for 2 h, and subsequently incubated in low-glucose (5.5 mM, LG) or high-glucose (25 mM, HG) medium for 1 h. Untreated cells (CON) were included as a control. (A) INS concentrations in the culture supernatant by ELISA. n = 3; ^##P < 0.01 vs. CON; *P < 0.05 vs. LPS; ^ΔP < 0.05, ^ΔΔP < 0.01 vs. LPS + APS-H. (B) Ratio of the INS concentration in HG medium to that in LG medium. n = 3, ^#,*^,ΔP < 0.05, ^ΔΔP < 0.01