Table 5 Chemical screening of fungal extracts by thin-layer chromatography
Identified genus | Internal strain no. | Visual observation | Visibility under UV light (254Â nm) | Visibility under UV light (365Â nm) | Visibility with spray reagent | Prospective compounds |
|---|---|---|---|---|---|---|
Pestalotia sp. | TMFE-1 | Â | Blue quenching Dark quenching | Blue Sky blue Greenish yellow | Dark purple | Steroids, Terpenoids Coumarin, Isocoumarin or their derivatives |
Colletotrichum sp. | TMFE-2 | Â | Blue quenching Dark quenching | Greenish yellow Blue Sky blue | Dark purple Bluish purple | Steroids, Terpenoids Coumarin, Isocoumarin or their derivatives |
Colletotrichum sp. | TMFE-3 | Â | Dark quenching | Greenish yellow Light yellow | Pink Dark purple | Flavonoids or their derivatives, Terpenoids |
Colletotrichum sp. | TMLE-2 | Â | Blue quenching Dark quenching | Violet Sky blue | Purple Bluish purple | Coumarin, Isocoumarin or their derivatives Terpenoids, Steroids |
Fusarium sp. | TMLE-3 | Â | Light quenching Dark quenching | Blue Red Yellow Sky blue Violet | Dark purple | Coumarins Anthocyanins Terpenoids Steroids |
Fusarium sp. | DSLE-1 | Â | Blue Sky blue Violet Dark quenching | Yellow Violet Blue | Dark purple | Coumarin, Isocoumarin or their derivatives Flavonoids or their derivatives, Terpenoids Steroids |
Fusarium sp. | DSLE-2 | Â | Dark quenching Light quenching | Blue | Dark purple Magenta | Terpenoids, Steroids Coumarin, Isocoumarin or their derivatives |
Fusarium sp. | DSLE-4 | Â | Dark quenching | Violet Blue | Pink | Terpenoids Steroids Anthraquinones Coumarin, Isocoumarin or their derivatives |
Fusarium sp. | DSBE-1 | Â | Dark quenching Dark blue Light sky blue | Light yellow Blue Sky blue | Dark purple | Coumarin, Isocoumarin or their derivatives Terpenoids Steroids |
Fusarium sp. | AHPE-3 | Â | Light sky blue Light blue quenching | Sky blue Dark blue | Dark purple | Coumarin, Isocoumarin or their derivatives, Terpenoids, Steroids |
Fusarium sp. | AHPE-4 | Pink Reddish purple | Light blue Dark quenching | Sky blue Dark blue | Dark purple | Coumarin, Isocoumarin or their derivatives Naphthoquinones, Anthraquinone derivatives Terpenoids, Steroids |
Colletotrichum sp. | AHLE-1 | Â | Dark quenching Sky blue | Light green Sky blue Blue | Pink Purple | Terpenoids Steroids Coumarin, Isocoumarin or their derivatives |
Colletotrichum sp. | AHLE-4 | Â | Dark quenching Sky blue | Light green, Sky blue, Blue | Dark purple | Terpenoids, Steroids Coumarin, Isocoumarin or their derivatives |