Fig. 6
Effects of the crude extract (BTL) and compound 8 from the leaves of Bauhinia thonningii and of hydrogen peroxide (H2O2) on the reactive oxygen species (ROS) production in CCRF-CEM cells upon 24 h treatment. IC50 values 14.23 µg/mL for BTL and 3.03 µM for compound 8. Control cells were treated with DMSO to a final concentration of 0.1%. The ROS level was determined by the combined 2´,7´-dichlorodihydrofluorescein diacetate staining and flow cytometry; (**): values are significantly different to those of untreated control (p < 0.05). Results display mean values ± SD of three assays. BTL slightly enhanced ROS production; compound 8 enhanced higher ROS production