Fig. 2

Effects of the crude extract (BTL) and compound 8 from the leaves of Bauhinia thonningii, and doxorubicin on the cycle distribution in CCRF-CEM cells after 24 h treatment. IC50 values 14.23 µg/mL for BTL, 3.03 µM for compound 8 and 0.02 µM for doxorubicin. Control cells were treated with DMSO to a final concentration of 0.1%. (**): values were significantly different to those of untreated control (p < 0.05); The propidium iodide (PI) staining was used for the cell cycle analysis by the flow cytometry; BTL, compound 8 and doxorubicin induced apoptosis in CCRF-CEM cells as shown with increase in cells in sub-G0/G1 phase. At higher concentration (2 × IC50), BTL induces cell cycle arrest in G0/G1 phase; compound 8 induced cell cycle arrest in G2/M phase; doxorubicin induced cell cycle arrest in G2/M and S phases