Fig. 9

Cellular apoptosis analysis in N-MT-treated HepG2 cells. HepG2 cells were treated by 2.3 and 3.1 µg/mL nano missile encapsulated milk thistle, and then, the apoptotic population was measured by the Annexin V-PI assay, using flow cytometry at 24h and 48h. A Flow cytometry of the apoptosis for untreated cells, B, C Flow cytometry of the apoptosis for N-MT-treated HepG2 cells at two different time points (24h and 48h) after treatment, and D Apoptotic cell percentage of total cells was increased at 24h, and 48h in HepG2 cell line. ∗  ∗ P < 0.01, ∗  ∗  ∗ P < 0.001