Skip to main content
Fig. 5 | BMC Complementary Medicine and Therapies

Fig. 5

From: Milk thistle nano-micelle formulation promotes cell cycle arrest and apoptosis in hepatocellular carcinoma cells through modulating miR-155-3p /SOCS2 /PHLDA1 signaling axis

Fig. 5

Real-time PCR analysis of three apoptosis-related genes after treating the cells with N-MT and MT in Huh-7 and HepG2 cells. Huh-7 cells were treated with 2 and 1.5 µg/mL N-MT and MT for 24h and 48h, respectively. HepG2 cells were treated with 2.3 and 3.1 µg/mL N-MT and MT for24h and 48h, respectively. A, B P21, (C, D) BAX, (E, F) BCL-2. The mRNA expression of the apoptosis-related genes was evaluated by the optimized real-time PCR method at 24h and 48h after treatment. The results are presented as the mean mRNA expression fold changes compared to the untreated. The analyses indicated that N-MT caused P21 and BAX mRNA level upregulation, while BCL-2 gene expression significantly decreased in both cell lines compared to the untreated cells. ∗ P < 0.05, ∗  ∗ P < 0.01, ∗  ∗  ∗ P < 0.001, ns: non-significant

Back to article page

BMC Complementary Medicine and Therapies

ISSN: 2662-7671

Contact us