Fig. 5

RRAGC mutation renders SUDHL-4 sensitive to cardamonin. Normal, RagC^WT and RagC^T90N-mutant HEK-293T or SUDHL-4 cells were treated with cardamonin (12 µM). (A) Immunoprecipitates pulled down by an anti-FLAG antibody were collected from cells expressing the indicated cDNAs. Cell lysates and immunoprecipitates were analysed by western blotting (n = 3). (B) Cell viability of normal, RagC^WT and RagC^T90N-mutant SUDHL-4 cells was determined by CCK-8 assay. Data are presented as mean ± SD (n = 3). ^**p < 0.01 compared with the control group; ^##p < 0.01 compared with the cardamonin-treated RagC^WT group. (C) RagC^WT and RagC^T90N-mutant SUDHL-4 cells were treated with cardamonin (12 µM) or everolimus (0.5 µM). Cell lysates were analysed by western blotting to measure the level of the indicated proteins (n = 3)