Fig. 4

Effects of the RagC^T90N mutant on mTORC1 signalling. HEK-293T or SUDHL-4 cells were transfected with lentiviruses expressing FLAG-GFP, FLAG-RagC^WT and FLAG-RagC^T90N. (A) Immunoprecipitates pulled down by an anti-FLAG antibody from cells expressing the indicated cDNAs were collected. Cell lysates and immunoprecipitates were analysed by western blotting (n = 3). (B, C) Cell viability of normal, FLAG-RagC^WT- and FLAG-RagC^T90N-expressing HEK-293T and SUDHL-4 cells was determined by CCK-8 assay. Data are presented as mean ± SD (n = 3). ^**p < 0.01 compared with the normal group; ^##p < 0.01 compared with the RagC^WT group