HPLC-ESI/MS-MS characterization of compounds in Dolomiaea costus extract and evaluation of cytotoxic and antiviral properties: molecular mechanisms underlying apoptosis-inducing effect on breast cancer

El-Nashar, Heba A. S.; Eldahshan, Omayma A.; Fattah, Nasra F Abdel; Loutfy, Samah A; Abdel-Salam, Ibrahim M

doi:10.1186/s12906-023-04164-9

BMC Complementary Medicine and Therapies

Table 1 Characteristics of twenty peaks from D. costus tentatively identified by HPLC/ESI-MS/MS in the negative and positive ionization mode

From: HPLC-ESI/MS-MS characterization of compounds in Dolomiaea costus extract and evaluation of cytotoxic and antiviral properties: molecular mechanisms underlying apoptosis-inducing effect on breast cancer

Peak No	R_t (min)	(M – H) ^– m/z	(M + H) ⁻ m/z	Molecular weight	Molecular formula	MS² fragments (m/z)	Tentative identification	Chemotaxonomy (Previous reported Species)	Class	Ref.
1.	0.22	245	247	246	C₁₃H₁₀O₅	232, 217	Isopimpinellin	Saussurea involucrata	Coumarin	[3]
2.	0.74	387	389	388	C₂₁H₂₄O₇	-	Medioresinol	Saussurea stella	Lignans	[1]
3.	1.04	339	-	340	C₁₅H₁₆O₉	178	Esculetin hexoside	Saussurea involucrata	Coumarin
4.	5.69	577	-	578	C₃₀H₂₆O₁₂	407, 289, 125	Procyanidin B2	Saussurea costus	Anthocyanidin	[7]
5.	6.67	475	477	476	C₂₂H₂₀O₁₂	300	Chrysoeriol-O-hexouronide	Saussurea costus	Flavonoid	[7]
6.	6.80	477	-	478	C₂₂H₂₂O₁₂	316	Nepetin-O-hexoside	Saussurea involucrata	Flavonoid	[44]
7.	6.91	-	419	418	C₂₂H₂₆O₈	387, 371, 327, 181	Syringaresinol	Saussurea stella	Lignan	[1]
8.	7.00	519	-	520	C₂₆H₃₂O₁₁	357, 327, 151	Pinoresinol-O-hexoside	Saussurea stella	Lignan	[1]
9.	7.04	623	-	624	C₂₈H₃₂O₁₆	315, 299	Isorhamnetin-O-hexoside-O-deoxyhexoside	Saussurea costus Saussurea involucrata	Flavonoid	[7, 44]
10.	7.14	-	233	232	C₁₅H₂₀O₂		Costunolide		Sesquiterpene lactone	[45]
11.	7.92	461	-	462	C₂₂H₂₂O₁₁	300, 283, 232, 148	Diosmetin-O-hexoside	Saussurea costus Saussurea stella	Flavonoid	[1, 7]
12.	9.15	431	433	432	C₂₁H₂₀O₁₀	269, 225, 151	Apigenin-O-hexoside	Saussurea involucrata	Flavonoid	[45]
13.	9.47	-	481	480	C₂₁H₂₀O₁₃	319	Gossypetin-O-hexoside	Saussurea costus	Flavonoid	[7]
14.	11.65	299	-	300	C₁₆H₁₂O₆	284	Hispidulin		Flavonoid	[46]
15.	13.30	591	-	592	C₂₈H₃₂O₁₄	284, 242	Acacetin-O-hexoside-O-deoxyhexoside	Saussurea costus	Flavonoid	[7]
16.	15.12	269	-	270	C₁₅H₁₀O₅	225, 165, 151	Apigenin	Saussurea costus	Flavonoid	[7]
17.	18.25	609	611	610	C₂₇H₃₀O₁₆	465, 300, 271	Quercetin-O-hexoside-O-deoxyhexoside (Rutin)	Saussurea lappa	Flavonoid	[45]
18.	19.26	301	303	302	C₁₅H₁₀O₆	240, 141	Hesperetin	Saussurea costus	Flavonoid	[7]
19.	25.42	-	611	610	C₂₇H₃₀O₁₆	448, 285,267, 175	Luteolin-di-O-hexoside	Saussurea costus	Flavonoid	[7]
20.	27.69	369	371	370	C₁₆H₁₈O₁₀	207, 192	Fraxetin-O-hexoside	Saussurea eopygmaea	Coumarin	[49]

Back to article page

ISSN: 2662-7671

Contact us

Submission enquiries: bmccomplementarymedicineandtherapies@biomedcentral.com
General enquiries: ORSupport@springernature.com