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Table 2 Multiple comparisons of fluorescence intensity of test materials with negative control and pairwise comparisons of fluorescence intensity of test materials and controls over seven days

From: Preliminary in vitro cytotoxic evaluation of Uncaria gambier (Hunt) Roxb extract as a potential herbal-based pulpotomy medicament

Time (day)

 

Test materials and controls

1000 μg/ml Ug

500 μg/ml Ug

250 μg/ml Ug

125 μg/ml Ug

62.5 μg/ml Ug

31.25 μg/ml Ug

15.6 μg/ml Ug

7.8 μg/ml Ug

MTA 48 h

MTA 4 h

Positive control

Negative control

1

FI (mean ± SD)

521.78 ± 4.09a

467.76 ± 18.08d

457.04 ± 32.80g

459.38 ± 9.40j

452.18 ± 16.69m

450.78 ± 17.21p

447.28 ± 28.90t

446.48 ± 16.90x

433.74 ± 15.13B

26.50 ± 2.77E

24.56 ± 7.64

442.32 ± 6.16F

Mean cell viability (%)

117.96

105.75

103.33

103.86

102.23

101.91

101.12

100.94

98.06

5.99

5.55

100.00

p-value

< 0.001

0.320

0.988

0.179

0.959

0.986

1.000

1.000

0.970

< 0.001

< 0.001

-

2

FI (mean ± SD)

592.10 ± 7.87b

755.96 ± 57.09e

769.24 ± 31.09h

766.58 ± 16.13k

774.22 ± 39.93n

773.22 ± 13.29q

781.44 ± 33.75u

778.74 ± 22.16y

746.7 ± 21.88C

31.80 ± 6.71

28.62 ± 3.19

792.04 ± 33.28G

Mean cell viability (%)

74.76

95.44

97.12

98.05

97.75

97.62

98.66

98.32

94.28

4.01

3.61

100.00

p-value

0.001

0.967

0.983

0.994

0.999

0.970

1.000

0.999

0.433

< 0.001

< 0.001

-

3

FI (mean ± SD)

535.34 ± 10.07a

866.08 ± 12.69f

956.96 ± 42.35i

986.56 ± 72.05l

988.56 ± 37.94o

1013.56 ± 21.29r

1010.96 ± 30.11v

1037.76 ± 33.76z

1010.36 ± 73.17D

35.42 ± 1.04F

30.40 ± 1.96

1043.96 ± 11.76H

Mean cell viability (%)

51.28

82.96

91.67

94.50

94.69

97.09

96.84

99.41

96.78

3.39

2.01

100.00

p-value

< 0.001

< 0.001

0.101

0.791

0.288

0.349

0.563

1.000

0.986

< 0.001

< 0.001

-

7

FI (mean ± SD)

644.42 ± 5.40c

785.60 ± 23.02e

1009.16 ± 21.38i

1024.56 ± 7.40l

1041.56 ± 5.59o

1075.16 ± 20.33s

1085.36 ± 16.95w

1089.76 ± 48.84A

775.24 ± 49.66C

24.76 ± 1.30E

25.38 ± 4.19

1081.96 ± 9.73I

Mean cell viability (%)

59.56

72.61

93.27

94.69

96.27

99.37

100.31

100.72

71.65

2.29

2.35

100.00

p-value

< 0.001

< 0.001

0.011

< 0.001

0.003

1.000

1.000

1.000

0.001

< 0.001

< 0.001

-

  1. Multiple comparisons with Games-Howell post hoc test for comparison of test materials with negative control, p is statistically significant at α = 0.05
  2. FI Fluorescence intensity, SD Standard deviation, Ug Uncaria gambir, MTA Mineral trioxide aggregate
  3. Pairwise comparisons with Bonferroni adjustment for comparison of test material at particular concentration over time; different letters (a vs b) in each column indicated significant different for test material at specific concentration over time (p < 0.05) while similar letters (a vs a) denote insignificant difference (p > 0.05)