Table 1 The information of primers used for real-time PCR.
From: Hesperetin attenuates the expression of markers of adipose tissue fibrosis in pre-adipocytes
Gene name | Gene acronym | Accession No. | Sequence | Product length |
|---|---|---|---|---|
collagen type VI | Col6a3 | XM_030245451.2 | 5’- AACCCTCCACATACTGCTAATTC-3’ 5’- TCGTTGTCACTGGCTTCATT-3’ | 70 |
Osteopontin (secreted phosphoprotein 1) | Opn (Spp1) | NM_001204203.1 | 5’- GCCTGTTTGGCATTGCCTCCTC-3’ 5’- CACAGCATTCTGTGGCGCAAGG-3’ | 158 |
matrix metallopeptidase 2 | Mmp2 | XM_006530751.4 | 5’- CAGGGAATGAGTACTGGGTCTATT-3’ 5’- ACTCCAGTTAAAGGCAGCATCTAC-3’ | 119 |
matrix metallopeptidase 9 | Mmp9 | NM_013599.5 | 5’- AATCTCTTCTAGAGACTGGGAAGGAG-3’ 5’- AGCTGATTGACTAAAGTAGCTGGA-3’ | 128 |
Beta-actin | Actb | NM_007393.5 | 5’- GTCCTCCTGGCATACCATAGA-3’ 5’- AGCTCAGTAACAGTCCGCCTAGA-3’ | 101 |