Fig. 11

Topo I inhibitory binding assay by agarose gel electrophoresis. Lane “D” represents pure pBR322 DNA (0.175 µg). “T” is the mixture of topo I (0.5 U) and pBR322 DNA (0.175 µg). The same amounts of topo I and DNA were added to all the other lanes. Lanes “A” represents the addition of ASH extract (2.0 µg), and that “C” indicates camptothecin (1.4 µg, positive control). Lanes “M”, “Q”, “I”, represent the addition of the mixture of 1.0 µg quercetin and 1.4 µg isorhamnetin, quercetin (1.0 µg), isorhamnetin (1.4 µg), respectively