Fig. 3
Representative dot plots of cell apoptosis: (A) HepG2 and (C) WI-38 cells treated with IC50/2 concentration of amygdalin or sorafenib alone and in combination for 48 h before double staining with annexin V-FITC/PI and analysis by flowcytometry. The percentage of live cells (AnnexinV-FITC− /PI −), early apoptotic (AnnexinV-FITC+ /PI −), late apoptotic (AnnexinV-FITC+ /PI +), and necrotic cells (AnnexinV-FITC− /PI +) were measured, represented graphically in (B) and (D). Data are represented as mean ± SEM (n = 3; *significantly different as compared to the untreated control group; *** (P ≤ 0.001), ** (P ≤ 0.01),* (P ≤ 0.005)