Fig. 6
Comparative metabolic flux analysis of MDA cells treated with frozen PJ. Cells were analyzed in microplates by Seahorse XP 96 according to the Mito Stress protocol as described in material and methods. a Statistical analysis of mitochondrial respiration stress test assay. The histograms show Basal, resting OCR; oligo, OCR in the presence of oligomycin; FCCP, OCR under uncoupled condition. Data, means ± SEM are expressed as percentage of control Basal OCR (0.56 ± 0.06 pmol O2/min /µg protein). b The histograms show ATP turnover (i.e., basal – oligomycin), maximal capacity (OCR in the presence of FCCP) and reserve capacity (i.e., “max” – basal). Data, means ± SEM are expressed as percentage of control Basal OCR (c) Statistical analysis of glycolytic activity stress assay. The histograms show basal glycolysis, glycolytic reserve (i.e., oligo – basal ECAR) and glycolytic capacity (i.e. oligo- 2Deoxy-glucose). Data, means ± SEM are expressed as percentage of control Basal ECAR (0.493 ± 0.05 mpH/min /μg protein) (d) Energy map was obtained plotting basal OCR versus glycolysis ECAR. Statistical significances are referred to control: * p < 0.05; ** p < 0.01; *** p < 0.001