Fig. 4

Effect of PJ from pomegranate stores in Controlled atmosphere (CA) and in regular air (RA) on mitochondrial function in MDA cells. Cells were incubated 24 h with PJ obtained from CA or RA stored pomegranate fruit for 30 or 60 days and and analyzed in microplates by Seahorse. a Statistical analysis of the OCRs obtained from Mitostress assay. The histograms show Basal, resting OCR; oligo, OCR in the presence of oligomycin; FCCP, OCR under uncoupled condition. Data, means ± SEM are expressed as percentage of control Basal OCR. b The histograms show ATP turnover, maximal capacity and reserve capacity. Data, means ± SEM are expressed as percentage of control Basal OCR. c Statistical analysis of glycolytic activity stress assay. The histograms show basal glycolysis, glycolytic reserve and glycolytic capacity. Data, means ± SEM are expressed as percentage of control Basal ECAR. d Energy map was obtained plotting basal OCR versus glycolysis ECAR; * p < 0.05, ** p < 0.01; *** p < 0.001. Statistical significances are referred to control