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Fig. 3 | BMC Complementary Medicine and Therapies

Fig. 3

From: Characterization of the effect of pomegranate crude extract, and its post-harvesting preservation procedures, on redox tone, cellular growth and metabolic profile of MDA-MB-231 cell line

Fig. 3

Effect of PJ processed with thermal pasteurization (PT) and high hydrostatic pressure (HHP) on mitochondrial function in MDA cells. MDA were incubated 24 h with fresh, thermal pasteurization (PT) or high pressure pasteurization (HPP) PJ and analyzed in microplates by Seahorse. a Statistical analysis of the OCRs obtained from Mitostress assay. The histograms show Basal, resting OCR; oligo, OCR in the presence of oligomycin; FCCP, OCR under uncoupled condition. Data, means ± SEM are expressed as percentage of control Basal OCR. b The histograms show ATP turnover, maximal capacity and reserve capacity. Data, means ± SEM are expressed as percentage of control Basal OCR (c) Statistical analysis of glycolytic activity stress assay. The histograms show basal glycolysis, glycolytic reserve and glycolytic capacity. Data, means ± SEM are expressed as percentage of control Basal ECAR. d Energy map was obtained plotting basal OCR versus glycolysis ECAR; * p < 0.05, ** p < 0.01; *** p < 0.001. Statistical significances are referred to control

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