Fig. 4

SAF-Hex induces apoptotic cell death in HeLa cells. HeLa cells were treated with SAF-Hex at concentrations of 0, 5, and 25 µg/mL for 36 h. A Western blotting was used to measure apoptosis-related protein expression. Original images of blots are shown in Fig. S3. B Relative ratio of BAX and BCL-2, as determined using Western blot results. C qPCR was utilized to determine the relative transcription levels of BAX and BCL2. qPCR, quantitative PCR; SAF-Hex, hexane fraction of the SAF extract. Data are represented as mean ± SD and analyzed using one-way ANOVA; *P < 0.05, **P < 0.01; ns, not significant; n = 3–4