Fig. 2

Effects of SAF-Hex on HeLa cell proliferation. HeLa cells were treated with SAF-Hex at concentrations of 0, 5, and 25 µg/mL for 36 h. A Western blotting was performed to assess the expression levels of proteins involved in the cell cycle and cell survival. Original images of blots are shown in Fig. S1. B Fold changes in expression of Cyclin E, CDK2, and CDK1. C Relative transcription levels of PCNA, as determined using quantitative PCR. SAF-Hex, hexane fraction of the SAF extract. Data are represented as mean ± SD and analyzed by one-way ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001; n = 3–4