Table 1 The most abundant compounds of the TFE identified by LC-MS.
Compounds | Subclass | mz | Retention time (s) | Mode | Relative abundance | Relative content (%) |
|---|---|---|---|---|---|---|
D-Galactose | Carbohydrates and carbohydrate conjugates | 179.056 | 91.8 | neg | 3.116E + 10 ± 6.95E + 08 | 15.27% |
L-Theanine | Amino acids, peptides, and analogues | 175.108 | 109.7 | pos | 2.192E + 10 ± 4.55E + 08 | 10.75% |
Catechin | Flavans | 289.071 | 414.1 | neg | 2.179E + 10 ± 3.24E + 09 | 10.68% |
Caffeine | Purines and purine derivatives | 195.086 | 438.5 | pos | 1.022E + 10 ± 9.3E + 09 | 5.01% |
L-Phenylalanine | Amino acids, peptides, and analogues | 166.085 | 302.4 | pos | 8.245E + 09 ± 3.78E + 08 | 4.04% |
L-Isoleucine | Amino acids, peptides, and analogues | 132.102 | 191.2 | pos | 6.873E + 09 ± 2.56E + 08 | 3.37% |
Astragalin | Flavonoid glycosides | 447.093 | 491.9 | neg | 5.245E + 09 ± 1.8E + 09 | 2.57% |
D-Fructose | Carbohydrates and carbohydrate conjugates | 179.056 | 103.2 | neg | 4.036E + 09 ± 3.71E + 09 | 1.98% |
Scopoletin | Hydroxycoumarins | 191.035 | 575 | neg | 4.377E + 09 ± 8.7E + 07 | 2.15% |
L-Glutamic acid | Amino acids, peptides, and analogues | 130.049 | 156.6 | pos | 3.53E + 09 ± 1.5E + 08 | 1.73% |
4-Hydroxycinnamoylagmatine | Hydroxycinnamic acids and derivatives | 276.144 | 243.6 | pos | 3.337E + 09 ± 7.5E + 08 | 1.64% |
D-Xylose | Carbohydrates and carbohydrate conjugates | 149.044 | 89.9 | neg | 3.328E + 09 ± 1.1E + 08 | 1.63% |
5-Aminopentanoic acid | Amino acids, peptides, and analogues | 118.085 | 104.5 | pos | 3.162E + 09 ± 9.7E + 07 | 1.55% |
Epigallocatechin gallate | Flavans | 457.077 | 416.7 | neg | 3.059E + 09 ± 2.2E + 08 | 1.50% |
Salicylic acid | Benzoic acids and derivatives | 137.023 | 350.5 | neg | 2.865E + 09 ± 5.4E + 07 | 1.40% |
2-Keto-glutaramic acid | Short-chain keto acids and derivatives | 145.048 | 94.3 | pos | 2.652E + 09 ± 6.1E + 0.8 | 1.30% |
Adenine | Purines and purine derivatives | 134.046 | 246 | neg | 2.502E + 09 ± 6E + 07 | 1.23% |
Threonic acid | Carbohydrates and carbohydrate conjugates | 135.029 | 82.4 | neg | 2.417E + 09 ± 9E + 07 | 1.18% |
3-Hydroxymethylglutaric acid | Fatty acids and conjugates | 145.049 | 283.6 | pos | 2.339E + 09 ± 3.5E + 08 | 1.15% |
(S)-2-Propylpiperidine | - | 128.143 | 836.8 | pos | 2.224E + 09 ± 1.1E + 0.8 | 1.09% |
Muramic acid | Carbohydrates and carbohydrate conjugates | 234.097 | 106.3 | pos | 2.11E + 09 ± 7.7E + 07 | 1.03% |
Pyroglutamic acid | Amino acids, peptides, and analogues | 128.036 | 89.7 | neg | 2.051E + 09 ± 1.1E + 0.8 | 1.01% |
L-Tyrosine | Amino acids, peptides, and analogues | 182.081 | 185.8 | pos | 1.979E + 09 ± 3.9E + 07 | 0.97% |
2-Ketobutyric acid | Short-chain keto acids and derivatives | 101.024 | 91.8 | neg | 1.828E + 09 ± 9.7E + 07 | 0.90% |