Table 1 Sequences of primers used for the real-time PCR analysis
Gene | Primers | Sequence |
|---|---|---|
Srebp-1c | Forward | 5′-CTGTTGGTGCTCGTCTCCT-3′ |
|  | Reverse | 5′-TTGCGATGCCTCCAGAAGTA-3′ |
Ppar-γ | Forward | 5′-GATGACAGCGACTTGGCAAT-3′ |
|  | Reverse | 5′-TGTAGCAGGTTGTCTTGAATGT-3′ |
Fas | Forward | 5′-ATCCTGGCTGACGAAGACTC-3′ |
|  | Reverse | 5′-TGCTGCTGAGGTTGGAGAG-3′ |
Acc | Forward | 5′-GATGAACCATCTCCGTTGGC-3′ |
|  | Reverse | 5′-CCCAATTATGAATCGGGAGTGC-3′ |
Cpt1 | Forward | 5′-CCAGGCTACAGTGGGACATT-3′ |
|  | Reverse | 5′-GAACTTGCCCATGTCCTTGT-3′ |
Ppar-α | Forward | 5′-GGAGCGTTGTCTGGAGGTT-3′ |
|  | Reverse | 5′-GAAGTGGTGGCTAAGTTGTTGA-3′ |
Mcad | Forward | 5′-AGGTTTCAAGATCGCAATGG-3′ |
|  | Reverse | 5′-CTCCTTGGTGCTCCACTAGC-3′ |
CD36 | Forward | 5′-ATGGGCTGTGATCGGAACTG-3′ |
|  | Reverse | 5′-GTCTTCCCAATAAGCATGTCTCC-3′ |
Fabp1 | Forward | 5′-ATGAACTTCTCCGGCAAGTACC-3′ |
|  | Reverse | 5′-CTGACACCCCCTTGATGTCC-3′ |
Fatp1 | Forward | 5′-CACGATCCCGTGCATCTTCC-3′ |
|  | Reverse | 5′-AGCATTGGAGTAGGTGTCCAG-3′ |
ApoB | Forward | 5′-TTGGCAAACTGCATAGCATCC-3′ |
|  | Reverse | 5′-TCAAATTGGGACTCTCCTTTAGC-3′ |
ApoE | Forward | 5′-CTCCCAAGTCACACAAGAACTG-3′ |
|  | Reverse | 5′-CCAGCTCCTTTTTGTAAGCCTTT-3′ |
Mttp | Forward | 5′-CTCTTGGCAGTGCTTTTTCTCT-3′ |
|  | Reverse | 5′-GAGCTTGTATAGCCGCTCATT-3′ |
β-actin | Forward | 5′-TGCTGTCCCTGTATGCCTCT-3′ |
|  | Reverse | 5′-TGATGTCACGCACGATTTCC-3′ |