Fig. 6From: Fumigaclavine C ameliorates liver steatosis by attenuating hepatic de novo lipogenesis via modulation of the RhoA/ROCK signaling pathwayFC suppresses Srebp-1c mediated hepatic DNL by modulating the RhoA/ROCK signaling pathway in FFA-induced AML-12 cell. A FC-treatment on AML-12 cell viability. B The level of hepatocellular TG was examined. C The levels of FPP and GGPP were measured by HPLC–MS/MS. D, E Western blotting technique was used to evaluate the expressions of prenyl-RhoA, ROCK, and Srebp-1c. FFA, free fatty acid; TG, Triglyceride; FPP, farnesyl diphosphonate; GGPP, geranylgeranyl diphosphonate; Srebp-1c, sterol response element binding protein-1c. Sim (20 μM) acted as the positive control group. Each value represents as means ± S.E.M. of triplicate experiments. # P < 0.01 as compared with the control group. * P < 0.05, ** P < 0.01, and *** P < 0.001 as compared with the FFA-induced groupBack to article page