Fig. 5
L.E.M. extract suppresses macrophage changes in cancer-bearing mice L.E.M. extract suppresses macrophage changes in cancer-bearing mice. B16F10 cells (7.5 × 105 cells) were injected subcutaneously into the left sole of the mice (WT group, N = 8; B16 group, N = 15; B16-L.E.M. group, N = 15). From Day 7 after administering the tumor cells, the mice were orally administered 200 µL of PBS or L.E.M. extract daily at a dose of 2 g/kg. Mice that showed tumor metastasis from the left sole to the thigh after tumor cell administration were euthanized (B16 group, N = 2; B16-L.E.M. group, N = 2). On Day 28 after administration, the mice were euthanized, the tumor weight was measured, and the spleen was recovered (WT group, n = 5; B16 group, n = 6; B16-L.E.M. group, n = 4). (a) Percentage of macrophages in the spleen measured by CD11b and F4/80 and analyzed by flow cytometry. Figures show the percentage of CD11b−F4/80+ cells, as well as the typical flow cytometry results. The data are representative of two independent experiments and were statistically analyzed by Student’s t-test or Welch’s t-test (N.S., not significant). (b) Proportion of CD86+ cells and CD80+ cells in CD11b−F4/80+ cells analyzed by flow cytometry. The median value is shown in the figure. The data are representative of two independent experiments and were statistically analyzed by Student’s t-test or Welch’s t-test (N.S., not significant)