Fig. 2

Chromatographic profile and analysis of caraway nsLTP1. a 2D-RP-HPLC elution profile of pooled gel filtration fractions containing caraway nsLTP1 on Aeris 3.6 µm WIDEPORE C4 250 × 4.6 mm column. b MALDI-TOF mass spectra of RP-HPLC purified caraway nsLTP1. c Amino acid sequence of nsLTP from caraway seeds. Solid line represent the N-terminal sequence of intact protein, while arrows represent cleaved peptides sequenced after trypsin and CNBr digestion