Table 1 Primers sequences for RT-PCR amplification
Gene name and symbol | Sequence (5′⟶ 3') | Amplicon size (bp) | TM | |
|---|---|---|---|---|
F | R | |||
Bcl-2 | F:5´-GGTGGGGTCATGTGTGTGG-3´ R:5´-CGGTTCAGGTACTCAGTCATCC-3´ | 89 | 60.6 | 60.1 |
BAD | F:5´-TGGACTCCTTTAAGAAGGGAC-3´ R:5´-CAAGTTCCGATCCCACCAG-3´ | 113 | 56.6 | 57.8 |
Fas R | F: 5´-AGCGCTGAAGAGCCAACATA-3´ R: 5´-TGGGTACTTAGCATGCCACT-3´ | 126 | 59.7 | 58.7 |
Caspase- 3 | F:5´-TGCCTGTAACTTGAGAGTAGATGG-3´ R:5´-CTTCACTTTCTTACTTGGCGATGG-3´ | 172 | 59.8 | 60.1 |
Caspase- 8 | F: 5-ACATGGACTGCTTCATCTGC-3´ R:5´-AAGGGCACTTCAAACCAGTG-3´ | 123 | 58.2 | 58.6 |
Caspase- 9 | F:5´-TGCTGCGTGGTGGTCATTCTC-3´ R:5´-CCGACACAGGGCATCCATCTG-3´ | 94 | 63.2 | 63.1 |
GAPDH | F:5’-AAGCTCATTTCCTGGTATGACAACG-3’ R:5’-TCTTCCTCTTGTGCTCTTGCTGG-3’ | 126 | 61.6 | 62.6 |