Fig. 5

Flow cytometry analysis of treated/untreated cancerous and normal cells. Cells were treated with FITC-Annexin V in combination with PI to detect apoptosis and necrosis before being subjected for analysis by flow cytometry. Panels represent untreated control groups, treated with BHE, treated with betanin and treated with 5-FU (105 μL/well of 6-well plate) as positive control groups for 48 h incubation, respectively. Dots with Annexin V-/PI + (Q1), Annexin V + /PI + (Q2), Annexin V + /PI- (Q3), and Annexin V-/PI (Q4) and feature represent necrotic, late apoptotic, early apoptotic, and viable intact cells, respectively