Comparative anticancer effects of Annona muricata Linn (Annonaceae) leaves and fruits on DMBA-induced breast cancer in female rats

Table 5 Effect of A. muricata on the relative weight of some organs and hematological parameters

Organs (mg/kg)	NOR	DMBA	TAMOX	AML	AMF_3 days	AMF_Daily
Thymus	1396.7 ± 40.3	768 ± 40.3###	1423.7 ± 20.9***	439.3 ± 27.1***	826.5 ± 54.3	1116.1 ± 35.1***
Liver	30,381 ± 624.7	35214.6 ± 1331.6##	30922.6 ± 235.4*	30794.4 ± 908.1**	33716.5 ± 1684.2	30970.6 ± 396.6*
Kidneys	5618 ± 113.6	5780.8 ± 322.3	5902.1 ± 83.6	5767 ± 136.3	5888.7 ± 115.6	5506.3 ± 80.9
Femur	2569.7 ± 84.7	2257 ± 106#	3044.2 ± 41.1***	3217.7 ± 92.5***	2960.1 ± 47.2	2673.1 ± 75.4**
Spleen	6000 ± 85.5	7127.4 ± 710.6#	4335.3 ± 44.5***	3413.2 ± 69.1***	4263.6 ± 180.1***	4148.1 ± 120.8***
Hematology
WBC (×10³/µl)	12.5 ± 0.54	15.42 ± 0.51##	12.8 ± 0.7	9.9 ± 1	351.2 ± 128.1***	6 ± 1.1
Lymphocytes (%)	69.8 ± 5	66 ± 0.4	79.7 ± 2.5*	73.7 ± 3.9	79.6 ± 3.4	70.2 ± 3.3
Monocytes (%)	5.2 ± 0.5	6.32 ± 0.9	4.6 ± 0.9	13.3 ± 4.2	24.4 ± 2.4***	15.6 ± 0.6**
Granulocytes (%)	18.4 ± 1.5	27.62 ± 1.34##	16.8 ± 1.4***	9.9 ± 1.3***	11.2 ± 1.2***	19.5 ± 0.4***
RBC (×10³/µl)	8 ± 0.5	5.58 ± 0.76#	7.8 ± 0.4*	6.6 ± 0.4	6.2 ± 0.2	7.1 ± 0.4*

Normal (NOR) and negative control (DMBA) groups received distilled water (vehicle); Tamox + DMBA = rats serving as positive control, treated with tamoxifen at 3.3 mg/kg BW; AML + DMBA = rats treated with the ethanolic extract of A. muricata leaves at 200 mg/kg BW; AMF = rats treated with A. muricata pulp fruit extract at 200 mg/kg BW at 2 different frequencies (3 times/week and every day). Significance compared to the NOR group: #p < 0.05, ##p < 0.01, ###p < 0.001. Significance compared to the DMBA group: *p < 0.05, **p < 0.01, ***p < 0.001

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