Fig. 3
From: Wound healing effects of Asparagus lucidus Lindl extract through the phosphorylation of ERK1/2
AL extract increases the expression of cell cycle regulatory genes by activating ERK1/2 in HaCaT cells. HaCaT cells were treated with the indicated concentration of AL extract. Total protein was used for Western blot analysis, and cDNA from total mRNA was used for qRT-PCR. A Phosphorylated forms of p38, ERK1/2, JNK, ERK5, and AKT were detected. GAPDH was used as a quantitative control. Full-length blots are presented in Supplementary Figure S1. B Quantity of p-ERK1/2 was calculated using densitometric analysis and normalized to GAPDH. C Changes in mRNA expression of gene encoding cyclins and CDKs. D Changes in mRNA expression of growth factors. E Changes in mRNA expression of AP-1 transcription factors and Elk1. (*p < 0.05, ** p < 0.01)