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Fig. 7 | BMC Complementary Medicine and Therapies

Fig. 7

From: Butyrate inhibits the mitochondrial complex Ι to mediate mitochondria-dependent apoptosis of cervical cancer cells

Fig. 7

Butyrate inhibited the mitochondrial complex Ι in CC cells. (A) CCK8 was used to determine the viability of HeLa cells treated with various concentrations of rotenone for 48 h. (B) Levels of mitochondrial NADH and NAD + treated with rotenone or butyrate for 48 h. (C) Flow cytometry for quantitative detection of ROS after rotenone treatment. (D) Western blot analysis for Caspase 3, cleaved-Caspase 3, Bax, Bcl-2, Caspase 9, cleaved-Caspase 9, Apaf-1 and (E) for mitochondrial complex Ι. (F) Quantitative analysis of Western blot in CON, Rot and NaB groups. CON: Control group. NaB: Group treated with 5mM butyrate for 48 h. Rot: Group treated with 0.2µM rotenone for 48 h. Data were expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001

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BMC Complementary Medicine and Therapies

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