Fig. 1From: Butyrate inhibits the mitochondrial complex Ι to mediate mitochondria-dependent apoptosis of cervical cancer cellsButyrate suppressed the proliferation of human CC cells. CCK8 was used to determinate the proliferation of (A) HeLa and (B) Ca Ski cells treated with 5mM butyrate for 12, 24, 48 and 72 h. The EdU staining assay was performed to detect apoptotic cells in (C) HeLa and (E) Ca Ski cells. (D, F) Quantitative analysis of the proportion of red fluorescence in EdU. (G) The cell cycle distribution of HeLa cells was analyzed by flow cytometry. (H) Quantitative analysis of S-phase in HeLa cells. (I) Western blot analysis of Cyclin D1. (J) Quantitative analysis of Western blot in CON and NaB groups. CON: Control group; NaB: Group treated with 5mM butyrate for 48 h. All data above are expressed as mean ± SD. * P < 0.05; *** P < 0.001Back to article page