Table 1 IC-values, SR, and P-values of single and combined treatment with Sora and Cur or Kmf in human colorectal cancer cell lines

A. Single and combined treatment with Sora and Cur.

IC-values (µM)

SR

P-value

IC-values (µM)

SR

P-value

1. Sequential treatment with Sora and Cur: Sora (24 h) followed by Cur (48 h)

 a. Sora (0.25- 10 µM)

IC₆₀ = 7.70

N.A

-

IC₇₀ = 0.86

N.A

-

 b. Sora (0.25- 10 µM) + Cur (60 µM)

IC₆₀ = 9.14

0.843

0.307

IC₇₀ = 0.86

1.00

0.993

 c. Sora (0.25- 10 µM) + Cur (120 µM)

IC₆₀ = 4.00

1.925

0.006

IC₇₀ = 0.57

1.51

0.457

2. Inverted sequential treatment with Sora and Cur: Cur (24 h) followed by Sora (48 h)

 a. Sora (0.25- 10 µM)

IC₇₀ = 6.00

N.A

-

IC₆₀ = 8.00

N.A

0.081 vs. b

0.001 vs. c

 b. Sora (0.25- 10 µM) + Cur (60 µM)

IC₇₀ = 6.86

0.880

0.504

N.D

N.D

b

 c. Sora (0.25- 10 µM) + Cur (120 µM

IC₇₀ = 4.86

1.240

0.504

IC₆₀ = 2.00

4.00

c

3. Simultaneous treatment with Sora and Cur: (72 h)

 a. Sora (0.25- 10 µM)

IC₈₀ = 4.86

N.A

-

IC₆₀ = 7.43

N.A

0.019 vs. b

0.027 vs. c

 b. Sora (0.25- 10 µM) + Cur (60 µM)

IC₈₀ = 0.57

8.530

0.136

N.D

N.D

b

 c. Sora (0.25- 10 µM) + Cur (120 µM

IC₈₀ = 0.57

8.530

0.088

N.D

N.D

c

B. Single and combined treatment with Sora and Kmf

IC-values (µM)

SR

P- value

IC-values (µM)

SR

P-value

1. Sequential treatment with Sora and Kmf: Sora (24 h) followed by Kmf (48 h)

 a. Sora (0.25- 10 µM)

IC₆₀ = 0.57

N.A

-

IC₆₀ = 7.43

N.A

0.716 vs. b

 b. Sora (0.25- 10 µM) + Kmf (60 µM)

IC₆₀ = 1.14

0.500

0.560

IC₆₀ = 9.43

0.79

b

 c. Sora (0.25- 10 µM) + Kmf (120 µM)

IC₆₀ = 1.14

0.500

0.675

IC₆₀ = 6.86

1.08

0.214

2. Inverted sequential treatment with Sora and Kmf: Kmf (24 h) followed by Sora (48 h)

 a. Sora (0.25- 10 µM)

IC₆₀ = 4.86

N.A

-

IC₆₀ = 7.43

N.A

0.010 vs. b

0.008 vs. c

 b. Sora (0.25- 10 µM) + Kmf (60 µM)

IC₆₀ = 4.00

1.22

0.148

N.D

N.D

b

 c. Sora (0.25- 10 µM) + Kmf (120 µM)

IC₆₀ = 2.00

2.43

0.134

N.D

N.D

c

3. simultaneous treatment with Sora and Kmf: (72 h)

 a. Sora (0.25- 10 µM)

IC₈₀ = 4.86

N.A

-

IC₆₀ = 7.14

N.A

0.009 vs. b

0.013 vs. c

 b. Sora (0.25- 10 µM) + Kmf (60 µM)

IC₈₀ = 0.57

8.53

0.513

N.D

N.D

b

 c. Sora (0.25- 10 µM) + Kmf (120 µM)

IC₈₀ = 0.57

8.53

0.152

N.D

N.D

c