Fig. 13

Western blot analysis of the levels of cell cycle and apoptosis-associated proteins in the human colon cancer cell line SW1116 treated with Sora, Cur, and their simultaneous combinations. Cells were treated with Sora (5 µM), Cur (200 µM), Cur (400 µM) or a simultaneous combination of Sora (5 µM) and Cur (200 or 400 µM) for 72 h. The levels of proteins associated with cell cycle and apoptosis control were analysed using western blot analysis. A Cell cycle-associated proteins and (B) apoptosis-associated proteins. -actin was used as an internal control. Sora, sorafenib; Cur, curcumin; UT, untreated; pRb, phosphor retinoblastoma protein; Bcl-xL, Bcl extra-large protein. Full-length blots/gels are presented in Supplementary Fig. 1 Aa-Af, Ba-Be. Each protein was subjected to two separate western blot analyses. Because of the results' similarity, the WB of every protein for each experiment has been merged into a composite figure and a representative figure was used for publication (Fig. 13 A, B)