Fig. 7

HQD downregulated the expression of ERK and promoted Rap1 binding to GTP. A. B. The protein expression levels of phospho-ERK in the kidney cortex by Western blot assay. C.D. In the kidney, the active-Rap1 protein was enriched using the active-rap1 pull-down kit, and alterations were detected by Western blot. Values are mean ± SEM (n = 3), *P < 0.05 vs. db/m, #P < 0.05 vs. db/db by one-way ANOVA with Tukey's post doc analysis