Tocotrienol-rich fraction reduces retinal inflammation and angiogenesis in rats with streptozotocin-induced diabetes

Table 1 Primer sequence for tested genes and housekeeping genes

Primer name	NCBI Reference Number	Gene Symbol	Sequence	Annealing temperature (°C)
Interleukin-1 beta	24,494	IL1B_F IL1B_R	CTCCATGAGCTTTGTACAAGG GGGGTTGACCATGTAGTCGT	53.4
Interleukin-6	24,498	IL6_F IL6_R	AAGAAAGACAAAGCCAGAGTC CACAAACTGATATGCTTAGGC	52.8
Tumor necrosis factor-alpha	24,835	TNFA_F TNFA_R	TCAGCCTCTTCTCATTCCTGC TTGGTGGTTTGCTACGACGTG	56.8
Inducible nitric oxide synthase	24,599	INOS_F INOS_R	CACCACCCTCCTTGTTCAAC CAATCCACAACTCGCTCCAA	56.0
Monocyte Chemoattractant Protein-1	24,770	MCP1_F MCP1_R	CTCAGCCAGATGCAGTTAATGC TTCTCCAGCCGACTCATTGG	54.5
Interferon-gamma	25,712	IFNG_F IFNG_R	TATGGAAGGAAAGAGCCTCC TCTGTGGGTTGTTCACCTCG	58.7
Vascular endothelial growth factor A	83,785	VEGFA_F VEGFA_R	CAGCGACAAGGCAGACTATT GTTGGCACGATTTAAGAGGG	55.0
Insulin-like growth factor-1	24,482	IGF1_F IGF1_R	GTGTCCGCTGCAAGCCTAC CAAGTGTACTTCCTTCTGAGTCTTGG	59.0
Glyceraldehyde 3-phosphate dehydrogenase	24,383	GAPDH_F GAPDH_R	CCATGGAGAAGGCTGGGG CAAAGTTGTCATGGATGACC	58.2
Hypoxanthine-guanine phosphoribosyltransferase	24,465	HPRT_F HPRT_R	GACCGGTTCTGTCATGTCG ACCTGGTTCATCATCACTAATCAC	55.7

ISSN: 2662-7671