Fig. 10
Effect of TRF on retinal HIF-1α expression in STZ-induced DR rats. A Representative immunostained retinal sections showing effect of TRF on retinal HIF-1α expression; (B) Quantitative expression of the effect of TRF on retinal HIF-1α positive nuclei per mm2 (n = 4); (C) Quantitative expression of the effect of TRF on retinal HIF-1α protein expression measured by ELISA (n = 8). Blue arrows: nuclei positively stained for HIF-1α. N: Normal rats with vehicle treatment, DV: Diabetic rats with vehicle treatment, DT: Diabetic rats with TRF treatment, GCL: Ganglion cell layer. Data was parametric and presented as mean ± SD, *p < 0.05, *** p < 0.001 (Scale bar: 50 μm) (Magnification 20X)