Fig. 6

Effect of PSS and PL exposure of 4 breast cancer cell lines on apoptosis and necrosis. Cisplatin was used as a positive control. A Histograms shown are representative of 4 biological replicates of the untreated cells and 72-h treatment of PSS, PL, and Cisplatin was performed in 4 breast cancer cell lines. Cells were co-stained with the Annexin V-DY-634 and propidium Iodide (PI), the single cell population in each quadrant was generated. Graphs shown percentage of apoptotic and necrotic cells from each treatment were also plotted B MCF7, C T47D, D MDA-MB-231, and E MDA-MB-468. The results are provided as mean ± SE; ^*, ^**, ^***, ^**** represented P ≤ 0.05, P ≤ 0.01, P ≤ 0.001, and P ≤ 0.0001 from statistical analysis using Unpaired t-test compared between the untreated cells among the same group