Fig. 7

Effects of baicalein on viral protein expression at the post-adsorption stage of ZIKV infection assessed by western blotting. A549 cells were infected with MOI 1 of ZIKV prior to treating infected cells with 100 µM baicalein at 0, 1, 3, 6, 9 h (0, + 1, +3, + 6, +9) after infection. Cells were incubated further until 24 h post infection. Total proteins were extracted from cells and viral protein expression was examined by western blotting. Viral structural (E) protein, viral non-structural (NS1) protein, and control GAPDH protein were analyzed. Intensity of the protein bands was quantitated and normalized with control GAPDH by the ImageJ version 1.52a. The expression levels are shown in fold-change over the untreated sample (B, C). All experiments were undertaken in triplicate. Error bars show mean ± SD and p-value compared to DMSO control of each time-point (* p-value < 0.05, ** p-value < 0.01). UT = untreated. Full uncropped blots of all replicates are available in the associated Supplementary materials