Fig. 4

KJG reduces neuroinflammation response by inhibiting TLR4 in LPS-induced mice. (a) The effect of KJG on the depressive behaviors induced by LPS in mice (5 weeks, n = 10). The sucrose preference test (SPT, first), the open field test (OFT, second), the Tail suspension test (TST, third), and the forced swimming test (FST, fourth). (b) Nissl staining and immunohistochemistry staining were performed after behavior tests (n = 3; Scale bar: 50 μm). (c) The levels of TNF-α, IL-6, and IL-1β in the serum of mice and their statistical analysis (n = 8). (d) WB analysis for TLR4, p-FOXO1, and FOXO1 in hippocampal tissues (n = 3). One-way ANOVA: # P < 0.05, ## P < 0.01, ### P < 0.001 vs. control group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. LPS group