Xiaoyaosan ameliorates depressive-like behavior and susceptibility to glucose intolerance in rat: involvement of LepR-STAT3/PI3K pathway in hypothalamic arcuate nucleus

Table 1 Sequence of oligonucleotides used for RT-qPCR

Gene	Forward primer	Reverse primer	Annealing temperatures (°C)
LepR	CCGAGAAGATCCCCGAGACA	ACTTCCCTCGAGGTCTGGTG	60
JAK2	GTGCGTGCGAGCGAAGATCC	ACTGCTGAATGAACCTGCGGAATC	60
STAT3	CCAGTCGTGGTGATCTCCAACATC	CAGGTTCCAATCGGAGGCTTAGTG	60
POMC	AGGCGTGCGGAGGAAGAGAC	GACTCGTTCTCGGCGACATTGG	60
AGRP	GCAGCAGACCGAGCAGAAGATG	GCACAGGTCGCAGCAAGGTAC	60
NPY	TGCTCGTGTGTTTGGGCATT	GATGTAGTGTCGCAGAGCGG	60
GAPDH	CCATTCTTCCACCTTTGAT	TGGTCCAGGGTTTCTTACT	58
GLUT4	AGCCAGCCTACGCCACCATAG	CAGCAGAGCCACCGTCATCAAG	60
IRS-1	AGCAACAGCAGCAGCAGTCTTC	ACTCTTCCGAGCCAGTCTCTTCTC	60
PI3K	AACTCGCCTCATAGCAGAGCAATG	TGGCACGCAGTCATGGTTGATC	60
AKT	GGCAGGAGGAGGAGACGATGG	TTCATGGTCACACGGTGCTTGG	60

ISSN: 2662-7671