Fig. 4

Combination treatment of methyl gallate and oxaliplatin augmented susceptibility of ovarian cancer cells to NK cell-mediated cytolysis. A OVCAR-5 cells were pretreated for 24 h with or without 20 μM oxaliplatin, 10 μM oxaliplatin, 0.1 mg/mL methyl gallate (MG) alone, or combination of 0.1 mg/mL MG and 10 μM oxaliplatin. B SK-OV-3 cells were pretreated for 48 h with or without 50 μM oxaliplatin, 25 μM oxaliplatin, 0.02 mg/mL methyl gallate (MG) alone, or combination of 0.02 mg/mL MG and 25 μM oxaliplatin. Cells were then co-cultured with NK-92 cells at the various E:T ratios. Experiment was performed in triplicates. Results from one representative experiment of three are shown and presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant