Fig. 3

Increased expression of stress ligands for NK cell receptors on ovarian cancer cells after treatment with (A) ethyl acetate fraction of L. indica, (B) methyl gallate, and (C) combination of methyl gallate and oxaliplatin. OVCAR-5 cells were treated for 48 h with or without (A) L. indica ethyl acetate fraction (EA, 0.3 mg/mL), (B) methyl gallate (MG, 0.1 mg/mL), or (C) combination of methyl gallate (MG, 0.1 mg/mL) and oxaliplatin (10 µM), and then phenotype analyzed by FACS for the indicated ligands of NK cells: (a) CD112, (b) CD115, (c) MIC-A/B, (d) ULBP-1, (e) ULBP-2, (f) ULBP-3, (g) DR4 (TRAIL-R1), and (h) DR5 (TRAIL-R2). The relative mean fluorescence intensities of each stress ligand were compared between untreated cells (blue bars) and treated cells (red bars), and results presented are mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ns, not significant