Fig. 1

L. indica leaf extract pretreated ovarian cancer cells showed enhanced sensitivity to cytolysis by activated NK cells. OVCAR-5 cells were pretreated overnight with or without 0.3 mg/mL (A) crude extract, (B) ethyl acetate fraction, (C) dichloromethane fraction, (D) water soluble fraction and (E) water insoluble fraction of L. indica. Subsequently cells were co-cultured with activated NK cells at the various E:T ratios. Cytotoxicity assay was determined by measuring the viability of PKH-26 labelled target cancer cells on 96-well plate. Experiment was performed in triplicates. Results from one representative experiment of three are shown and presented as mean ± SD. Blue line represents untreated cells, while red line represents treated cells. ***p < 0.001; ns, not significant